Rat Liver Glutamyl Ribonucleic Acid Synthetase
نویسندگان
چکیده
منابع مشابه
Rat liver glutamyl ribonucleic acid synthetase. I. Purification and evidence for separate enzymes for glutamic acid and glutamine.
Glutamyl ribonucleic acid synthetase was purified approximately 150-fold from homogenates of rat liver to a specific activity of 60 mpmoles of glutamyl-RNA formed per min per mg of protein. The enzyme at this stage of purification was judged to be about 40% pure, but in a state of aggregation. Although the enzyme was quite stable to storage, it was very heat labile, and lost activity during its...
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It is generally accepted that in the first phase of protein biosynthesis, amino acid-specific enzymes, the aminoacyl-ribonucleic acid synthetases, carry out the activation of the various amino acids, and that the exacting specificity of these enzymes extends to the transfer of the amino acid to a particular soluble ribonucleic acid. Several investigators (l-5) have achieved the purification of ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96154-5